교수진 소개 │ 교육 │ GCSP

교수진

교수진 소개

Byung Il LEE

Name: Byung Il LEE, Ph.D.(adjunct professor)
Faculty Appointment (title, department): Professor, Department of Cancer Biomedical Science, Chief Scientist, Therapeutic Target Discovery Branch, Division of Precision medicine
Area of Expertise: Structural biology, Biochemistry, Structure Based Drug Discovery
E-mail: bilee@ncc.re.kr

Work Experience: Chief Scientist, National Cancer Center Korea (2016~ Present)
Principal Investigator, National Cancer Center Korea (2007~ Present)
Research Fellow, Dana-Farber Cancer Institute & Harvard Medical School, Boston, MA, USA (2005~2007)
Educational Background: 1994-1998, BS, Chemistry, Seoul National University
1998-2000, MS, Chemistry (Biochemistry), Seoul National University
2000-2004, Ph.D., Chemistry (Biochemistry), Seoul National University
Research Interests: My research focus on structural studies on apoptosis related proteins and protein kinases using X-ray crystallography. Apoptosis inhibitor proteins basically inhibit cell death by inhibiting the caspases. We recently determined structure of non-canonical IAP protein and found new mechanisms in apoptosis. Protein kinases are functions on lots of cell signaling and lots of protein kinases are therapeutic targets for cancers. So, for the innovative drug discovery, we are currently conducting structural studies on protein kinase structures and exploring new drug candidates through in silico or HTS chemical library screening.
International Collaboration
Achievements: 2020 SCI Regulation of mRNA export through API5 and nuclear FGF2 interaction. Nucleic Acids Res. Online published (11.147)

2019 SCI-E Gossypol Suppresses Growth of Temozolomide-Resistant Glioblastoma Tumor Spheres. Biomolecules. 10;9(10). (4.694)

2019 SCI-E Zwitterionic near-infrared fluorophore-conjugated epidermal growth factor for fast, real-time, and target-cell-specific cancer imaging. Theranostics. 9(4):1085-1095. (8.063)

2019 SCI A NCAPG2-Derived Phosphopeptide Selectively Binds to the Polo-Box Domain of PLK1 and Inhibits Cancer Cell Proliferation. Int J Pept Res Ther 25:1397-1403. (1.219)

2018 SCI Farnesyl diphosphate synthase is important for the maintenance of glioblastoma stemness. Exp Mol Med. 50(10):1-12. (5.584)

2018 Crystallization and preliminary X-ray analysis of API5-FGF2 complex: BioDesign. 6(4):92~95.

2018 SCI Crystal Structures of Spleen Tyrosine Kinase in Complex with Two Novel 4-Aminopyrido[4,3-d] Pyrimidine Derivative Inhibitors. Mol Cells.41(6):545-552. (3.533)

2017 SCI Sporadic Early-onset Diffuse Gastric Cancers Have High Frequency of Somatic CDH1 Alterations but Low Frequency of Somatic RHOA Mutations Compared with Late-onset Cancers: Gastroenterology. 153(2):536~549 (18.392)

2017 SCI-E Daurinol blocks breast and lung cancer metastasis and development by inhibition of focal adhesion kinase (FAK): Oncotarget. 8(34):57058~57071 (5.168)

2017 SCI Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2.: EMBO reports. 18(5):733~744 (8.568)

2017 SCI-E Antigen-responsive molecular sensor enables real-time tumor-specific imaging: Theranostics. 7(4):952~961 (8.712)

2017 SCI-E API5 confers cancer stem cell-like properties through the FGF2-NANOG axis: Oncogenesis. 6(1):e285~e285 (4.143)

2016 SCI-E Oncogenic function and clinical implications of SLC3A2-NRG1 fusion in invasive mucinous adenocarcinoma of the lung: Oncotarget. 7(43):69450~69465 (5.008)

2016 SCI Crystal structures of spleen tyrosine kinase in complex with novel inhibitors: structural insights for design of anticancer drugs: FEBS Journal. 283(19):3613~3625 (4.237)

2016 SCI-E Clinically Significant Unclassified Variants in BRCA1 and BRCA2 Genes Among Korean Breast Cancer Patients: Cancer Research and Treatment. Online:Published (4.245)

2016 SCI-E Crystal Structure of Human Myotubularin-Related Protein 1 Provides Insight into the Structural Basis of Substrate Specificity.: PLoS One. 11:e0152611~e0152611 (3.057)

2015 SCI Highly potent and selective pyrazolylpyrimidines as Syk kinase inhibitors: Bioorganic & Medicinal Chemistry Letters. 25:4441~4446 (2.42)

2015 SCI The Cell Shape-determining Csd6 Protein from Helicobacter pylori Constitutes a New Family of l,d-Carboxypeptidase.: The Journal of biological chemistry. 290:25103~25117 (4.573)

2015 SCI Structural and biochemical insights into the role of testis-expressed gene 14 (TEX14) in forming the stable intercellular bridges of germ cells: Proceedings of the National Academy of Sciences of the USA. 112:12372~12377 (9.674)

2015 기타 Recombinant Human Laforin Expressed in Insect Cells: Expression, Purification, and Biochemical Characterizations: Journal of the Korean Chemical Society. 59:466~470 (0)

2015 SCI Structure and mechanism of activity-based inhibition of the EGF receptor by Mig6: Nature Structural & Molecular Biology. 22:703~711 (13.309)

2015 SCI-E Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1: Acta crystallographica. Section F, Structural biology communications. 71(Pt3):261~265 (0.527)

2015 기타 Crystal structure of a class 2 D-xylose isomerase from the human intestinal tract microbe Bacteroides thetaiotaomicron: BioDesign. 3:41~47 (0)

2015 SCI Structure of Csd3 from Helicobacter pylori, a cell shape-determining metallopeptidase: Acta crystallographica. Section D, Biological crystallography. 71(Pt3):675~686 (2.674)

2014 SCI Structural basis for the recognition of muramyltripeptide by Helicobacter pylori Csd4, a D,L-carboxypeptidase controlling the helical cell shape: Acta crystallographica. Section D, Biological crystallography. 70(pt11):2800~2812 (7.232)

2014 SCI-E Overproduction, crystallization and preliminary X-ray crystallographic analysis of Escherichia coli tRNA N(6)-threonylcarbamoyladenosine dehydratase: Acta crystallographica. Section F, Structural biology communications. 70(Pt11):1517~1520 (0.568)

2014 SCI The condensin component NCAPG2 regulates microtubule-kinetochore attachment through recruitment of Polo-like kinase 1 to kinetochores.: Nature Communications. 5:4588~4588 (10.742)

2014 SCI Novel 3-arylethynyl-substituted thieno[3,4-b]pyrazine derivatives as human transglutaminase 2 inhibitors: Organic and biomolecular chemistry. 12(27):4932~4940 (3.487)

2013 SCI Identification of Potent Inhibitors against Human Peptide Deformylase as Anticancer Agents: Bulletin of the Korean Chemical Society. 34:3885~3887 (0.982)

2013 SCI-E Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. F69:1127~1130 (0.552)

2013 SCI-E Crystal Structure of Pim1 Kinase in Complex with a Pyrido[4,3-D]Pyrimidine Derivative Suggests a Unique Binding Mode: PLoS One. 8(7):e70358~e70358 (3.73)

2012 SCI Structures of Staphylococcus aureus peptide deformylase in complex with two classes of new inhibitors: Acta Crystallographica Section D: Biological Crystallography. 68(7):784~793 (12.619)

2012 SCI Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family: FEBS Letters. 586(9):1384~1388 (3.538)

2012 SCI Helical Repeat Structure of Apoptosis Inhibitor 5 Reveals Protein-Protein Interaction Modules: JOURNAL OF BIOLOGICAL CHEMISTRY. 287(14):10727~10737 (4.773)

2012 SCI Direct characterization of protein oligomers and their quaternary structures by single-molecule FRET: Chemical Communications. 48(8):1138~1140 (6.169)

2011 SCI Quantitative measurement of anti-aquaporin-4 antibodies by enzyme-linked immunosorbent assay using purified recombinant human aquaporin-4: Multiple Sclerosis. 18(5):578~586 (4.255)

2011 SCI Cancer cells promote survival through depletion of the von Hippel Lindau tumor suppressor by protein crosslinking: Oncogene. 30(48):4780~4790 (7.414)

2011 SCI Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily: Journal of Structural Biology. 175:329~338 (3.5)

2011 SCI-E Overexpression, crystallization and preliminary X-ray crystallographic analysis of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family: Acta Crystallographica Section F. 67(Pt8):930~932 (0.563)

2011 SCI Crystal structure of the catalytic domain of cholesterol-a-glucosyltransferase from Helicobacter pylori: PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS. 79(7):2321~2326 (2.813)

2011 SCI Pyrogallin, an ATP-Competitive Inhibitor of JAK3: Bulletin of the Korean Chemical Society. 32:1077~1079 (0.871)

2010 SCI Helicobacter pylori proinflammatory protein upregulates NF-κB as a cell-translocating Ser/Thr kinase: Proceedings of the National Academy of Sciences USA (PNAS). 107(50):21418~21423 (9.432)

2010 SCI I-kappaBalpha depletion by transglutaminase 2 and mu-calpain occurs in parallel with the ubiquitin-proteasome pathway: Biochemical and biophysical research communications. 399(2):300~306 (2.548)

2010 SCI Crystal structure of human transglutaminase 2 in complex with adenosine triphosphate: International journal of biological macromolecules. ;47(2):190~195 (2.366)

2010 SCI Refined Structure of Human NM23-H1 from a Hexagonal Crystal: Bulletin of the Koeran Chemical Society. 31:1397~1399 (0.936)

2010 SCI Expression, purification and biochemical characterization of the N-terminal regions of human TIG3 and HRASLS3 proteins.: Protein Expression and Purification. 71(1):103~107 (1.563)

2010 SCI-E Antinociceptive effects of Alpinia katsumadai via cyclooxygenase-2 inhibition: Biomolecules & Therapeutics. 18:159~165 (0.292)

2010 SCI The mechanism of transglutaminase 2 inhibition with glucosamine: implications of a possible anti-inflammatory effect through transglutaminase inhibition: Journal of Cancer Research and Clinical Oncology. 136(1):143~150 (2.261)

2009 SCI Crystal Structure of the TNF-α-Inducing Protein (Tipα) from Helicobacter pylori: Insights into Its DNA-Binding Activity: J Mol Biol.. 392(1):191~197 (4.146)

2009 SCI Depletion of nucleophosmin via transglutaminase 2 cross-linking increases drug resistance in cancer cells: Cancer Letters. 274:201~207 (3.504)

2008 SCI Molecular modeling and biochemical studies of transglutaminase 2 mutation found in patients with early-onset type 2 diabetes: Bulletin of the Korean Chemical Society. 29:1797~1799 (1.156)

2007 SCI Crystal structure of human nucleophosmin-core reveals plasticity of the pentamer-pentamer interface: Proteins. 69:672~678 ()

2006 SCI Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa: Biochem Biophys Res Commun. 340:1028~1038 ()

2005 SCI Crystallization and preliminary X-ray crystallographic analysis of UDP-N-acetylglucosamine enolpyruvyl transferase from Haemophilus influenzae in complex with UDP-N-acetylglucosamine and fosfomycin: Mol Cells. 19:398~401 ()

2004 SCI Crystal Structure of the Schiff Base Intermediate Prior to Decarboxylation in the Catalytic Cycle of Aspartate α-Decarboxylase: J. Mol. Biol.. 340:1~7 (5.229)

2004 SCI Ring-shaped architecture of RecR: Implications for its role in homologous recombinational DNA repair: EMBO J.. 23:2029~2038 (10.053)

2004 SCI Crystallization and preliminary X-ray crystallographic analysis of nicotinic acid mononucleotide adenylyltransferase from Pseudomonas aeruginosa.: Acta Crystallogr. D. 60:948~949 (1.401)

2004 SCI Crystal structure of chorismate synthase complex with FMN: A novel fold and functional insights: J. Mol. Biol.. 336:904~915 (5.229)

2004 SCI Crystallization and preliminary X-ray crystallographic analysis of recombinational DNA repair protein RecR from Deinoccus radiodurans: Acta Crystallogr. D. 60:379~381 (1.401)

2004 SCI Crystal structure of ß-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase: J. Mol. Biol.. 335:155~165 (5.229)

2003 SCI Crystal structure of the UDP-N-acetylglucosamine acyltransferase from Helicobacter pylori: Proteins. 53:772~774 (4.684)

2003 SCI Crystal structure of tRNA(m(1)G37)methyltransferase: insights into tRNA recognition: EMBO J.. 22:2593~2603 (10.053)

2003 SCI Crystal structure of the type II 3-dehydroquinase from Helicobacter pylori: Proteins. 51:616~617 (4.684)

2003 SCI Crystal structure of class I acetohydroxy Acid isomeroreductase from Pseudomonas aeruginosa: J. Mol. Biol.. 328:505~515 (5.229)

2003 SCI Crystallization and preliminary X-ray crystallographic studies of chorismate synthase from Helicobacter pylori: Acta Crystallogr. D. 328:505~515 (1.401)

2003 SCI The TB structural genomics consortium: a resource for Mycobacterium tuberculosis biology: Tuberculosis. 83:223~249 (3.899)

2002 SCI Crystallization and preliminary X-ray crystallographic analysis of acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa: Acta Crystallogr. D. 58:2145~2146 (1.401)

2002 SCI Crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Pseudomonas aeruginosa: Acta Crystallogr. D. 58:1874~1875 (1.401)

2002 SCI Crystallization and preliminary X-ray crystallographic analysis of enoyl-acyl carrier protein reductase from from Helicobacter pylori: Acta Crystallgr. D. 58:1071~1073 (1.401)

2002 SCI Crystallization and preliminary X-ray crystallographic analysis of UDP-N-acetyl glucosamine acyltransferase from Helicobacter pylori: Acta Crystallgr. D. 58:864~866 (1.401)

2002 SCI Crystallization and preliminary X-ray crystallographic analysis of Aspartate 1-decarboxylase from from Helicobacter pylori: Acta Crystallgr. D. 58:861~863 (1.401)

2002 SCI Crystallization and preliminary X-ray crystallographic analysis of ß-xylosidase from Thermoanaerobacterium saccharolyticum, a?thermophilic anaerobe: Acta Crystallgr. D. 58:531~532 (1.401)

2002 SCI Crystallization and preliminary X-ray crystallographic analysis of the Rv2002 gene product from Mycobacterium tuberculosis, a ß-ketoacyl?carrier protein reductase homologue: Acta Crystallgr. D. 58:303~305 (1.401)

2001 SCI Crystallization and preliminary X-ray crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Saccharomyces cerevisiae: Acta Crystallogr. D. 57:1147~1149 (1.401)

2001 SCI Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii,a novel member of lactate/malate family of dehydrogenases: J. Mol. Biol.. 307:1351~1362 (5.229)

2001 SCI Crystallization and preliminary X-ray crystallographic analysis of the TM1442 gene product from Thermotoga maritima, a homolog of Bacillus subtilis anti-anti-sigma factor: Acta Crystallogr. D. 57:276~278 (1.401)

2000 SCI Lactate dehydorgenase from the hyperthermophilic archaeon Methanococcus jannaschii: Overexpression,Crystallization,and Preliminary X-ray analysis: Acta Crystallogr. D. 56:81~83 (1.401)